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African swine fever virus is harmless to humans, but pigs aren’t so lucky. The virus, endemic to warthogs, bushpigs and soft ticks in sub-Saharan Africa, causes a hemorrhagic fever in domestic swine.

D. CHARRO, G. ANDR脡S, N.G. ABRESCIA

African swine fever currently poses a monumental threat to the pork industries of Central and East Asia.

Since making its way to the Republic of Georgia in 2007 via the pork trade, a strain of the virus has swept through China and much of Southeast Asia, killing hundreds of thousands of pigs directly and nearly 200 million more by culling. Although a number of promising vaccine candidates are being developed, they still and only protect against the appropriately named Georgia 2007 strain, while many equally deadly strains circulate in Africa.

The virus’ unique multilayered architecture may be blocking vaccine development. , and their colleagues recently peered between those membranes to capture the structure of ASFV’s virion. They in the Journal of Biological Chemistry.

“What was striking is that actually you have four layers: two envelopes and two protein capsids for this virus,” said Abrescia, a structural biologist at the Center for Cooperative Research in Biosciences near Bilbao, Spain. “So you have this outer envelope which is acquired when the virus goes out of a cell, but below this envelope you have another set of icosahedral capsids and in between a membrane bilayer, all of them enclosing the genome-containing nucleoid.”

In previous studies, Andr茅s found that both the extracellular and intracellular forms of ASFV are infectious, which suggested that the outer membrane might not be necessary for infectivity, as the virus sheds its coat while entering into a host cell. The researchers hope that a better understanding of ASFV’s structure might contribute to more efficient vaccine design.

Abrescia, who examines how the structure and assembly of viruses with complex membranes affect pathogenicity, began collaborating with Andr茅s after becoming intrigued by ASFV’s unique double lipoprotein membranes and outer capsid protein fold. Andr茅s, a senior researcher at the ’s Electron Microscopy Unit in Madrid, set his sights on the virus while working on his doctoral thesis in the early 1990s during the height of the Spanish government’s efforts to rid the Iberian Peninsula of African swine fever.

COURTESY OF NICOLA ABRESCIA

Nicola G. A. Abrescia is a principal investigator in the Structure and Cell Biology of Viruses Lab at the Centre for Cooperative Research in Biosciences.

Long before Georgia 2007 appeared, another strain of ASFV arrived in Portugal in 1957. From Lisbon, the virus crept across the country and reached Spain in 1960; it leaped across that country as pigs were shuffled between . With the aid of the soft tick Ornithodoros erraticus, the virus became entrenched in Spain’s population of wild boars, which mingle often with domestic pigs. For the next two decades, African swine fever flared up in Western Europe, the Caribbean and Brazil. By 1985, pork producers and the Spanish government had had enough and embarked on a successful 10-year campaign of sanitation, detection and eradication. Andr茅s has been probing ASFV’s structure and assembly pathway ever since.

One challenge of working with ASFV is the virus’ size. ASFV is big — a giant virus, in fact, almost twice the size of influenza virions and thicker than the typical layer of ice in which particles are suspended for cryo-electron microscopy. This complicates sample preparation. Additionally, most microscopes’ field of view can capture only a few virus particles, challenging efficient data collection. And despite its size, ASFV’s outer membrane isn’t resilient outside of a host.

“The outer lipid envelope is relatively fragile,” Abrescia said, “so you have to set up a very good purification protocol which doesn’t destroy so much of the virus’s envelope.”

Once the researchers had optimized their protocol, they went to co-author ’s lab at the University of Leiden in the Netherlands to use the institution’s Titan/Krios transmission electron microscope, collecting cryo-images of a thousand viral particles. The virus’ size also causes its capsids to span more than one focal plane, contributing to image blur. To tackle this, Abrescia and Andr茅s biochemically deconstructed the virus in solution and then purified and resolved the fold of the individual protein composing the outer capsid.

Abrescia’s team isn’t alone in pursuing the structure of ASFV. A few days before their JBC paper appeared online in October, a group at the Chinese Academy of Sciences in Beijing described the pathogen’s structure at greater resolution in the journal , and in late November, a third group at the University of Science and Technology of China another cryo-EM analysis of ASFV in the journal Cell Host & Microbe.

But despite the success of these groups, and a fourth group who the structure of ASFV’s major capsid protein p72 in the journal Cell Research in September, the inner capsid remains a challenge.

“Even the high-resolution structures that our Chinese colleagues have obtained do not fully explain the inner capsid,” Abrescia said. “Indeed, it has remained something that requires more exploration.”